"""Plots about the feature space itself."""
from __future__ import annotations
from typing import TYPE_CHECKING
import numpy as np
import pandas as pd
from mantispy._core._corr import corr_matrix
from mantispy._core._reduce import get_matrix
from mantispy._core.frames import as_frame
from mantispy._core.masks import feature_mask
from mantispy.pl._common import axes as _axes
from mantispy.pl._common import maybe_interactive as _maybe_interactive
if TYPE_CHECKING:
from anndata import AnnData
from matplotlib.axes import Axes
[docs]
def feature_correlation(
adata: AnnData,
key: str | None = "selected",
groupby: str = "feature_group",
max_features: int = 300,
ax: Axes | None = None,
) -> Axes:
"""Correlation heatmap with features ordered by their annotation.
Features are sorted by ``groupby`` and then by channel, with a line at each group boundary.
Ordering by annotation instead of clustering shows directly whether correlated features fall within the same measurement family.
Args:
adata: Object to draw.
Usually well-level profiles.
key: Restrict to features flagged by this boolean ``var`` column.
``None``, or a column the object does not hold, uses every feature.
groupby: ``var`` column to order and delimit by.
max_features: Draw at most this many features, taken in the sorted order.
ax: Axes to draw into.
Returns:
The axes drawn on, holding the correlation matrix on a diverging scale fixed to ``[-1, 1]``, with a line at each group boundary and one tick per group.
Raises:
KeyError: ``var`` has no ``groupby`` column, or no ``channel`` column.
"""
mask = feature_mask(adata, key)
annotation = as_frame(adata.var).loc[mask, [groupby, "channel"]].astype(str)
order = annotation.sort_values([groupby, "channel"]).index[:max_features]
positions = adata.var_names.get_indexer(order)
correlation = corr_matrix(get_matrix(adata)[:, positions])
ax = _axes(ax, (7, 6))
display = np.nan_to_num(correlation, nan=0.0)
image = ax.imshow(display, cmap="RdBu_r", vmin=-1, vmax=1)
labels = as_frame(adata.var).loc[order, groupby].astype(str).to_numpy()
boundaries = np.flatnonzero(labels[1:] != labels[:-1]) + 0.5
for boundary in boundaries:
ax.axhline(boundary, color="black", lw=0.5)
ax.axvline(boundary, color="black", lw=0.5)
centres = np.concatenate([[0], boundaries, [len(labels)]])
ticks = (centres[:-1] + centres[1:]) / 2
ax.set_xticks(ticks)
ax.set_xticklabels(pd.unique(labels), rotation=90, fontsize=7)
ax.set_yticks(ticks)
ax.set_yticklabels(pd.unique(labels), fontsize=7)
ax.set_title(f"feature correlation ({len(order)} features)", fontsize=9)
ax.figure.colorbar(image, ax=ax, fraction=0.04, label="correlation")
features = [str(name) for name in order]
_maybe_interactive(
"heatmap",
ax=ax,
matrix=display,
rows=features,
columns=features,
value_label="correlation",
title=f"feature correlation ({len(order)} features)",
)
return ax
[docs]
def feature_groups(adata: AnnData, key: str | None = None, ax: Axes | None = None) -> Axes:
"""How many features each group contributes, split by channel.
Pass ``key="selected"`` after feature selection to see which families survived.
Args:
adata: Object to draw.
key: Restrict to features flagged by this boolean ``var`` column.
``None``, or a column the object does not hold, uses every feature.
ax: Axes to draw into.
Returns:
The axes drawn on, with one stacked bar per feature group and one segment per channel.
Raises:
KeyError: ``var`` has no ``feature_group`` column, or no ``channel`` column.
"""
mask = feature_mask(adata, key)
# A geometry feature has no channel; count it under "none" rather than let value_counts drop the NaN.
annotation = as_frame(adata.var).loc[mask, ["feature_group", "channel"]].astype(object).fillna("none").astype(str)
counts = annotation.value_counts().unstack(fill_value=0)
# Colocalization features carry a pipe-joined channel pair, so they are pooled into one "multiple" channel.
combined = [channel for channel in counts.columns if "|" in channel]
if combined:
counts = counts.drop(columns=combined).assign(multiple=counts[combined].sum(axis=1))
order = [c for c in ["none"] if c in counts.columns]
order += sorted(c for c in counts.columns if c not in ("none", "multiple"))
order += [c for c in ["multiple"] if c in counts.columns]
counts = counts[order]
ax = _axes(ax, (7, 4))
counts.plot.bar(stacked=True, ax=ax)
ax.set_ylabel("features")
ax.set_xlabel("feature group")
ax.legend(title="channel", fontsize=7, title_fontsize=8, loc="upper left", bbox_to_anchor=(1.0, 1.0), frameon=False)
ax.tick_params(axis="x", rotation=45)
tidy = counts.reset_index().melt(id_vars="feature_group", var_name="channel", value_name="features")
_maybe_interactive(
"barh", ax=ax, data=tidy, x="features", y="feature_group", color="channel", title="feature groups"
)
return ax
