Plotting#
Plotting functions return Matplotlib axes and do not modify the object.
Plates and quality control#
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Well-grid heatmap of |
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Distribution of cells per well, split by |
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Per-feature distributions, before and after normalization when |
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Fraction of missing values per feature, per plate. |
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Two-by-two summary of the QC metrics |
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Row and column medians per plate, for spotting plate position artifacts. |
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Image quality score per image, with the flagged images marked. |
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Control wells projected onto principal components fitted on the controls alone. |
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Outlier score distribution, and the flagged fraction per |
Features#
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Correlation heatmap with features ordered by their annotation. |
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How many features each group contributes, split by channel. |
Replicates and reproducibility#
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Mean average precision against significance, with the strongest groups labeled. |
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Observed replicate correlation against each group's permutation threshold. |
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Profile-by-profile similarity, ordered by |
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Settings against settings: which plates, batches or laboratories agree with each other. |
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Agreement per perturbation, ranked, with the ones that reproduce colored. |
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The saturation curve with its spread across draws. |
Batch correction#
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R^2 of each principal component on each covariate. |
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Grouped bars of an |
Hits and effect sizes#
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Distance from the controls against significance, with the most distant groups labeled. |
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The largest effects for one group, colored by feature family. |
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Effect against significance, per feature, for one group. |
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Heatmap of perturbations by feature families. |
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Distance from the controls against viability, with the suspect groups marked. |
Dose response#
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One compound's response against dose, with the fitted curve when there is one. |
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One compound's ladder, with the background banded by what each concentration is doing. |
Mechanism of action#
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The confusion matrix of |
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Which mechanisms one profile's neighborhood is enriched for. |
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The group-by-group distance matrix, ordered so related groups sit together. |
Feature sets#
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Mean enrichment score per group per feature set. |
Clusters and gene sets#
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Draw the hierarchical clustering tree stored by |
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Coherence per gene set, the significant ones marked. |
Single cells#
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Stacked bars of cell-state fractions, averaged within each group. |
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Log DNA intensity per group, colored by assigned phase. |
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Local cell density against a feature, per group. |
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Cluster by group heatmap of significance, so an effect in one cell state stands out. |